The subject of this study is an analysis of the organizational culture in terms of innovation in a selected manufacturing enterprise. Organizational culture can inspire or hinder innovative processes in an enterprise. The first part of this paper, based on literature studies, defines organizational culture and innovation concepts and describes the characteristics of the types of cultures. In the remainder of this paper, the results of research conducted using the OCAI questionnaire (Cameron and Quinn’s model of competing values) in a particular enterprise were presented, showing the association of the dominating organizational culture with readiness for change.