Purpose: The purpose of this article is to explore the relationship between the organizational climate and the motivation and efficiency of work in agile development teams. The article describes the concept of organizational climate, its factors and its impact on motivation and efficiency in those teams. Design: The study was conducted in five development teams that operate both in enterprises whose main activity is based on software development and in enterprises where the teams develop software only for the internal use of the company. These teams are using agile software development methodologies. The empirical research was carried out using a questionnaire among members of those teams. Findings: Standard motivating factors are no longer sufficient to motivate and retain the company programmers. They value more independence in the tasks performed and want to have a real impact on the company’s operations and the atmosphere in the workplace. Organizational climate factors contribute to this commonly understood work atmosphere. These factors can be created by the organization to achieve the greatest possible motivation of employees, and therefore their efficiency. It is worth noting that agile software development methodologies determine the intensity of some of these factors. Value: Appropriate measurement and metrics of projects, processes, and IT teams allows us to determine whether they work optimally and to improve their efficiency. Motivated, creative and satisfied employees, who identify with the company, and consequently are efficient, are the keys to success.