This study explores the key factors influencing the motivation of knowledge workers (KWs) in Pakistan’s high-tech industry. As knowledge-intensive roles become increasingly vital in today’s competitive business landscape, understanding the motivators driving KWs is crucial for enhancing organizational performance and talent retention. Grounded in self-determination theory (SDT), this research examines the impact of five core motivational factors – autonomy, meaningful work, learning and development, recognition of achievement, and physical work environment – on KW motivation. Using a structured questionnaire, data were collected from 301 KWs across various roles in Pakistan’s high-tech sector. Structural equation modeling (SEM) was employed to analyze the relationships among these factors. The findings reveal that all five motivational elements significantly and positively influence KW motivation, with learning and development emerging as the strongest predictor. These results contribute to the existing literature by offering empirical evidence on KW motivation within an emerging economy context. The study also provides practical insights for managers and policymakers seeking to foster an environment that enhances engagement, innovation, and productivity among KWs.