Research on innovation indicates that due to the turbulent environment innovative processes may no longer be limited only to the use of internal knowledge developed through R&D processes. Enterprise should also focus on searching for exogenous sources of information in order to maximise their efficiency. This approach is referred to as open innovation. The aim of the article is to reduce the knowledge gap about the impact of organizational culture on open innovation practices. Many studies indicate, that opening the innovative processes requires a specific organizational culture that is also one of the key determinants of a company's response to changes in its environment. Organizational culture can also be one of the major barriers to the ability to implement open innovation in the company.